Springer Protocols: Abstract: Proteomics of Cerebrospinal Fluid: Methods for Sample Processing

Proteomics of Cerebrospinal Fluid: Methods for Sample Processing

By: John E. Hale³, Valentina Gelfanova³, Jin-Sam You⁴, Michael D. Knierman³, Robert A. Dean³

Abstract

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Cerebrospinal fluid (CSF) provides an important source of potential biomarkers for brain disorders and therapeutic drug development. Applications of proteomic technology to the identification and quantification of proteins in CSF are increasing rapidly. Key to obtaining reproducible and reliable data about protein levels in CSF are standardization of methods for sample collection, storage, and subsequent sample processing. Methods are described here for all steps of sample processing for a number of different proteomic approaches.

Affiliation(s): (3) Lilly Research Laboratories, Greenfield, Indiana
(4) Indiana Centers for Applied Protein Sciences, Indianapolis, Indiana

Book Title: 2D PAGE: Sample Preparation and Fractionation

Series: Methods in Molecular Biology | Volume: 425 | Pub. Date: Jan-25-2008 | Page Range: 53-66 | DOI: 10.1007/978-1-60327-210-0_5

Subject: Protein Science

Key Words: Cerebrospinal fluid - mass spectrometry - proteomics - silver staining - two-dimensional gel electrophoresis

References

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