Springer Protocols: Abstract: Albumin and Immunoglobulin Depletion of Human Plasma

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Albumin and Immunoglobulin Depletion of Human Plasma

By: Rosalind E. Jenkins³, Neil R. Kitteringham³, Carrie Greenough³, B. Kevin Park³

Abstract

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Plasma and serum have been the focus of intense study in recent years in the expectation that they will provide important biomarkers of health and disease, without the need for invasive procedures. This aim has been hindered by the fact that a few highly abundant proteins dominate the protein profile, masking the lower abundance proteins and limiting our ability to analyse the entire plasma proteome. This chapter details a simple and effective method for removal of two of the most dominant proteins in plasma, albumin and immunoglobulin.

Affiliation(s): (3) University of Liverpool, Liverpool, Great Britain

Book Title: 2D PAGE: Sample Preparation and Fractionation

Series: Methods in Molecular Biology | Volume: 425 | Pub. Date: Jan-25-2008 | Page Range: 15-25 | DOI: 10.1007/978-1-60327-210-0_2

Subject: Protein Science

Key Words: Affinity chromatography - albumin - depletion - immunoglobulin - plasma proteome

References

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