Springer Protocols: Abstract: Metalloproteomics in the Molecular Study of Cell Physiology and Disease

Metalloproteomics in the Molecular Study of Cell Physiology and Disease

By: Hermann-Josef Thierse³, Stefanie Helm³, Patrick Pankert³

Abstract

Full Text

Download PDF (387K)

Physical and chemical stresses as well as metal-related diseases can disrupt the normal trafficking of metal ions. Moreover, homeostatic imbalance of such metal ions may modulate essential cellular functions (including signal transduction pathways), may catalyze oxidative damage, and may affect the folding of nascent proteins. Here we describe a new qualitative subproteomic method for the detection, isolation, and identification of metal-interacting proteins. Combining both classical immobilized metal ion affinity chromatography (IMAC) and modern proteomic techniques (e.g., two dimensional gel electrophoresis [2-DE]), metal-specific proteins have been successfully isolated and identified to define a metalloproteome. These metal-specific proteomes may give new insights into metal-related pathophysiological processes, such as the allergic reaction to nickel, which represents the most common form of human contact hypersensitivity.

Affiliation(s): (3) University of Heidelberg, Mannheim, Germany

Book Title: 2D PAGE: Sample Preparation and Fractionation

Series: Methods in Molecular Biology | Volume: 425 | Pub. Date: Jan-25-2008 | Page Range: 139-147 | DOI: 10.1007/978-1-60327-210-0_12

Subject: Protein Science

Key Words: 2-DE; 2-dimensional gel electrophoresis - disease proteomics - IMAC - immobilized metal ion affinity chromatography - metal affinity - metalloproteome - nickel - nickel allergy - nitrilotriacetic acid

References

Download References

1.	Porath, J., Carlsson, J., Olsson, I., and Belfrage, G. (1975) Metal chelate affinity chromatography, a new approach to protein fractionation. Nature. 258, 598–9.

2.	Porath, J. (1992) Immobilized metal ion affinity chromatography. Protein Expr. Purif. 3, 263–81.

3.	Mondal, K. and Gupta, M. N. (2006) The affinity concept in bioseparation: evolving paradigms and expanding range of applications. Biomol. Eng. 23, 59–76.

4.	Sun, X., Chiu, J. F., and He, Q. Y. (2005) Application of immobilized metal affinity chromatography in proteomics. Expert Rev. Proteomics. 2, 649–57.

5.	Ueda, E. K., Gout, P. W., and Morganti, L. (2003) Current and prospective applications of metal ion-protein binding. J. Chromatogr. A. 988, 1–23.

6.	Hochuli, E., Dobeli, H., and Schacher, A. (1987) New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues. J. Chromatogr. 411, 177–84.

7.	Terpe, K. (2003) Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Appl. Microbiol. Biotechnol. 60, 523–33.

8.	Harlow, E., and Lane, D. (1988) Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

9.	Schmitt, J., Hess, H., and Stunnenberg, H. G. (1993) Affinity purification of histidine-tagged proteins. Mol. Biol. Rep.18, 223–30.

10.	Corthals, G. L., Aebersold, R., and Goodlett, D. R. (2005) Identification of phosphorylation sites using microimmobilized metal affinity chromatography. Methods Enzymol. 405, 66–81.

11.	Bollen, M. and Beullens, M. (2002) Signaling by protein phosphatases in the nucleus. Trends Cell Biol. 12, 138–45.

12.	Zhou, W., Merrick, B. A., Khaledi, M. G., and Tomer, K. B. (2000) Detection and sequencing of phosphopeptides affinity bound to immobilized metal ion beads by matrix-assisted laser desorption/ionization mass spectrometry. J. Am. Soc. Mass Spectrom. 11, 273–82.

13.	Hata, K., Morisaka, H., Hara, K., et al. (2006) Two-dimensional HPLC on-line analysis of phosphopeptides using titania and monolithic columns. Anal. Biochem. 350, 292–7.

14.	Larsen, M. R., Thingholm, T. E., Jensen, O. N., Roepstorff, P., and Jorgensen, T. J. (2005) Highly selective enrichment of phosphorylated peptides from peptide mixtures using titanium dioxide microcolumns. Mol. Cell Proteomics. 4, 873–86.

15.	Wolschin, F., Wienkoop, S., and Weckwerth, W. (2005) Enrichment of phosphorylated proteins and peptides from complex mixtures using metal oxide/hydroxide affinity chromatography (MOAC). Proteomics. 5, 4389–97.

16.	Gorg, A., Obermaier, C., Boguth, G., et al.(2000) The current state of two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis. 21, 1037–53.

17.	Gorg, A., Weiss, W., and Dunn, M. J. (2004) Current two-dimensional electrophoresis technology for proteomics. Proteomics. 4, 3665–85.

18.	Klose, J. (1975) Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues. A novel approach to testing for induced point mutations in mammals. Humangenetik. 26, 231–43.

19.	O’Farrell, P. H. (1975) High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250, 4007–21.

20.	Thierse, H. J., Moulon, C., Allespach, Y., et al. (2004) Metal-protein complex-mediated transport and delivery of Ni2+ to TCR/MHC contact sites in nickel-specific human T cell activation. J. Immunol. 172, 1926–34.

21.	Heiss, K., Junkes, C., Guerreiro, N., , et al. (2005) Subproteomic analysis of metal-interacting proteins in human B cells. Proteomics. 5, 3614–22.

22.	She, Y. M., Narindrasorasak, S., Yang, S., Spitale, N., Roberts, E. A., and Sarkar, B. (2003) Identification of metal-binding proteins in human hepatoma lines by immobilized metal affinity chromatography and mass spectrometry. Mol. Cell. Proteomics. 2, 1306–18.

23.	Smith, S. D., She, Y. M., Roberts, E. A., and Sarkar, B. (2004) Using immobilized metal affinity chromatography, two-dimensional electrophoresis and mass spectrometry to identify hepatocellular proteins with copper-binding ability. J. Proteome Res. 3, 834–40.

24.	Kulkarni, P. P., She, Y. M., Smith, S. D., Roberts, E. A., and Sarkar, B. (2006) Proteomics of metal transport and metal-associated diseases. Chemistry. 12, 2410–22.

25.	Martin, S. F., Merfort, I., and Thierse, H. J. (2006) Interactions of chemicals and metal ions with proteins and role for immune responses. Mini Rev. Med. Chem. 6, 247–55.

26.	Jungblut, P., Baumeister, H., and Klose, J. (1993) Classification of mouse liver proteins by immobilized metal affinity chromatography and two-dimensional electrophoresis. Electrophoresis. 14, 638–43.

Comments (Loading...)

Post comment/View all comments