Springer Protocols: Abstract: Sample Preparation of Culture Medium from Madin-Darby Canine Kidney Cells

Sample Preparation of Culture Medium from Madin-Darby Canine Kidney Cells

By: Daniel Ambort³, Daniel Lottaz³, Erwin Sterchi³

Abstract

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A reproducible, standardized and simple sample preparation methodology is the key to successful two-dimensional gel electrophoresis (2-DE). This chapter describes step-by-step the sample preparation of culture medium from Madin-Darby canine kidney (MDCK) cells. Tips and tricks are given to circumvent possible pitfalls.

Affiliation(s): (3) University of Berne, Berne, Switzerland

Book Title: 2D PAGE: Sample Preparation and Fractionation

Series: Methods in Molecular Biology | Volume: 425 | Pub. Date: Jan-25-2008 | Page Range: 113-130 | DOI: 10.1007/978-1-60327-210-0_10

Subject: Protein Science

Key Words: Bicinchoninic acid (BCA) assay - culture medium (CM) - isoelectric focusing (IEF) - Madin-Darby canine kidney (MDCK) - rehydration loading - two-dimensional gel electrophoresis (2-DE) ultracentrifugation - ultrafiltration

References

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