SpringerProtocols: Abstract: Comparative Genomic Hybridization on Spotted Oligonucleotide Microarrays

3. Comparative Genomic Hybridization on Spotted Oligonucleotide Microarrays

By: Young H. Kim², Jonathan R. Pollack²

Abstract

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Recent advances in DNA microarray technology have enabled researchers to comprehensively characterize the complex genomes of higher eukaryotic organisms at an unprecedented level of detail. Array-based comparative genomic hybridization (Array-CGH) has been widely used for detecting DNA copy number alterations on a genomic scale, where the mapping resolution is limited only by the number of probes on the DNA microarray. In this chapter, we present a validated protocol utilizing print-tip spotted HEEBO (Human Exonic Evidence Based Oligonucleotide) microarrays for conducting array-CGH using as little as 25 ng of genomic DNA from a wide variety of sources, including cultured cell lines and clinical specimens, with high spatial resolution and array-to-array reproducibility.

Affiliation(s): (2) Department of Pathology, Stanford University, Stanford, CA, USA

Book Title: Microarray Analysis of the Physical Genome: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 556 | Pub. Date: Feb-01-2009 | Page Range: 21-32 | DOI: 10.1007/978-1-60327-192-9_3

Subject: Genetics/Genomics

Key Words: DNA microarray - array-CGH - comparative genomic hybridization - HEEBO - post-processing - epoxysilane - whole-genome amplification

References

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