Springer Protocols: Abstract: Colony Forming Unit Assays for MSCs

Colony Forming Unit Assays for MSCs

By: Radhika Pochampally³

Abstract

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MSCs are the plastic adherent adult stem/progenitor cells from bone marrow originally referred to as fibroblastoid colony forming units, then in the hematological literature as marrow stromal cells, subsequently as mesenchymal stem cells, and most recently as multipotential mesenchymal stromal cells. MSCs were originally referred to as fibroblastoid colony-forming-cells because one of their characteristic features is adherence to tissue culture plastic and generation of colonies when plated at low densities ( 1,2 ). The efficiency with which they form colonies still remains an important assay for the quality of cell preparations. This chapter describes 2 methods to assay the colony forming ability of MSCs; (a) a traditional assay for colony forming units (CFUs) and (b) single-cell colony forming unit assay (Sc-CFU).

Affiliation(s): (3) Center for Gene Therapy and Department of Pharmacology, Tulane University Health Sciences Center, New Orleans, LA

Book Title: Mesenchymal Stem Cells: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 449 | Pub. Date: Apr-01-2008 | Page Range: 83-91 | DOI: 10.1007/978-1-60327-169-1_6

Subject: Cell Biology

Key Words: Keywords - esenchymal stem cells - marrow stroma cells - multipotential stromal cells - single-cell colony forming unit assay

References

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