Springer Protocols: Abstract: Assays of MSCs with Microarrays

Assays of MSCs with Microarrays

By: Joni Ylőstalo³, Radhika Pochampally⁴, Darwin J. Prockop⁵

Abstract

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The rapid development of microarray technology during the last decade has greatly expanded the ability to define the genes expressed in cells. This chapter will focus on describing the steps required for conducting successful microarray experiments with multipotential stromal cells (MSCs). A complete microarray experiment, using the Affymetrix system, will be described starting from experimental design and ending with examples of data analysis using the dChip program.

Affiliation(s): (3) Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, LA
(4) Center for Gene Therapy and Department of Pharmacology, Tulane University Health Sciences Center, New Orleans, LA
(5) Center for Gene Therapy and Department of Biochemistry, Tulane University Health Sciences Center, New Orleans, LA

Book Title: Mesenchymal Stem Cells: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 449 | Pub. Date: Apr-01-2008 | Page Range: 133-151 | DOI: 10.1007/978-1-60327-169-1_10

Subject: Cell Biology

Key Words: Multipotential stromal cells - MSCs - microarray - gene expression - RNA - real-time PCR

References

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