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9. Enumeration of Bacteriophages Using the Small Drop Plaque Assay System
| Abstract |
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The determination of the concentration of infectious phage particles is fundamental to many protocols in phage biology, genetics,
and molecular biology. Described here is a drop plaque assay, which, being simpler, faster and more efficient than either
the classical overlay or direct plating methods, enhances efficiency in processing large numbers of samples.
Affiliation(s): (4) Public Health Agency of Canada, Laboratory for Foodborne Diseases, Guelph, Ontario, Canada
(5) Pro-Lab Diagnostics, Richmond Hill, Ontario, Canada
(5) Pro-Lab Diagnostics, Richmond Hill, Ontario, Canada
Book Title: Bacteriophages: Methods and Protocols, Volume 1: Isolation, Characterization, and Interactions
Series: Methods in Molecular Biology | Volume: 501 | Pub. Date: Jul-01-2008 | Page Range: 81-85 | DOI: 10.1007/978-1-60327-164-6_9
Subject: Microbiology
Key Words: Plaque - overlay technique - plaque-forming unit - pfu - plaque morphology - direct plating plaque assay - drop plaque assay - spot plaque assay
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