Most bacterial cells carry prophage genomes either integrated into the host DNA or present as repressed plasmids. Methods are described for the induction of prophages using Mitomycin C, and for the isolation of prophage-cured bacterial cell lines.
Book Title: Bacteriophages : Methods and Protocols, Volume 1: Isolation, Characterization, and Interactions
Series: Methods in Molecular Biology | Volume: 501 | Pub. Date: Jul-01-2008 | Page Range: 23-32 | DOI: 10.1007/978-1-60327-164-6_3
Key Words: Lysogen - induction - lytic cycle - lysogenic cycle - prophage - integration - SOS response - mitomycin - RecA - repressor