SpringerProtocols: Abstract: 12. Basic Phage Electron Microscopy

12. Basic Phage Electron Microscopy

By: Hans-W. Ackermann⁴

Abstract

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Negative staining of purified viruses is the most important electron microscopical technique in virology. The principal stains are phosphotungstate and uranyl acetate, both of which have problems and advantages. Particular problems are encountered in photography, calibration of magnification, measurements, and interpretation of artifacts.

Affiliation(s): (4) Department of Medical Microbiology, Faculty of Medicine, Laval University, Quebec, QC, Canada

Book Title: Bacteriophages: Methods and Protocols, Volume 1: Isolation, Characterization, and Interactions

Series: Methods in Molecular Biology | Volume: 501 | Pub. Date: Jul-01-2008 | Page Range: 113-126 | DOI: 10.1007/978-1-60327-164-6_12

Subject: Microbiology

Key Words: Artifacts - contrast - magnification - negative staining - positive staining - phosphotungstate - uranyl acetate

References

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