SpringerProtocols: Abstract: 11. Enumeration of Bacteriophages Using Flow Cytometry

11. Enumeration of Bacteriophages Using Flow Cytometry

By: Corina P.D. Brussaard⁴

Abstract

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Rapid identification and enumeration of the numerically important bacteriophages has been till recently a major limitation for studies of virus ecology. The development of sensitive nucleic acid stains, in combination with flow cytometric techniques, has changed this. The flow cytometric method allows the detection and discrimination of a wide variety of viruses of different morphology, genome type, and size. The present paper describes an optimized protocol for the enumeration of bacteriophages using a standard benchtop flow cytometer.

Affiliation(s): (4) Department of Biological Oceanography, Royal, Netherlands Institute for Sea Research, Texel, The Netherlands

Book Title: Bacteriophages: Methods and Protocols, Volume 1: Isolation, Characterization, and Interactions

Series: Methods in Molecular Biology | Volume: 501 | Pub. Date: Jul-01-2008 | Page Range: 97-111 | DOI: 10.1007/978-1-60327-164-6_11

Subject: Microbiology

Key Words: Bacteriophage - enumeration - detection - flow cytometry - green fluorescence - nucleic acid-specific staining - SYBR Green

References

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