SpringerProtocols: Abstract: 6. Candida Biofilm Analysis in the Artificial Throat Using FISH

6. Candida Biofilm Analysis in the Artificial Throat Using FISH

By: Bastiaan P. Krom³, Kevin Buijssen⁴, Henk J. Busscher³, Henny C. van der Mei³

Abstract

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Biofilm formation is a common complication of the use of prosthetic devices. In clinical settings, biofilms can be comprised of one or more microbial species. In order to investigate the interaction between different species within a biofilm, a reproducible, reliable model system has to be utilized and an appropriate system for species identification applied. The present chapter describes the artificial throat model, a model system for growing mixed species biofilms on shunt prostheses. The model is used in conjugation with fluorescent in situ hybridization (FISH), which facilitates identification and localization of the resident microorganisms within biofilms.

Affiliation(s): (3) Department of Biomedical Engineering, University Medical Center Groningen and the University of Groningen, Groningen, The Netherlands
(4) Department of Biomedical Engineering and Departments of Otorhinolaryngology, University Medical Center Groningen and the University of Groningen, Groningen, The Netherlands

Book Title: Candida albicans: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 499 | Pub. Date: Sep-01-2008 | Page Range: 45-54 | DOI: 10.1007/978-1-60327-151-6_6

Subject: Infectious Diseases

Key Words: Artificial throat - shunt prosthesis - mixed-species biofilm - microbial interactions - fluorescent in situ hybridization - FISH

References

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