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Microarrays—Analysis of Signaling Pathways

By: Anassuya Ramachandran³, Michael A. Black⁴, Andrew N. Shelling³, Donald R. Love⁵

Abstract

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Microarrays provide a powerful means of analyzing the expression level of multiple transcripts in two sample populations. In this study, we have used microarray technology to identify genes that are differentially regulated in response to activin-treated ovarian cancer cells. We find a number of biologically relevant genes that are involved in regulating activin signaling and genes potentially contributing to activin-mediated growth arrest appear to be differentially regulated. Thus, microarrays are an important tool for dissecting gene expression changes in normal physiological processes and disease.

Affiliation(s): (3) Department of Obstetrics and Gynaecology, Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealand
(4) Department of Biochemistry, University of Otago, Dunedin, New Zealand
(5) School of Biological Sciences, University of Auckland, Auckland, New Zealand

Book Title: Clinical Bioinformatics

Series: Methods in Molecular Medicine | Volume: 141 | Pub. Date: Dec-21-2007 | Page Range: 115-130 | DOI: 10.1007/978-1-60327-148-6_7

Subject: Bioinformatics

Key Words: Activin - Affymetrix - bioconductor - microarrays - quantitative real-time RT PCR - signaling pathways

References

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