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Computer-Assisted Reading of DNA Sequences
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DNA sequencing is increasingly used in a range of medical activities involving DNA diagnostics and research. This is the result
of improving technology and cheaper costs. Paradoxically, a greater demand for DNA sequencing has placed additional work on
the laboratory because sequencing profiles must be checked visually despite the availability of informatics-based tools in
interpreting DNA sequence traces. In this environment it is essential to have more sophisticated software that will allow
the sites of known and unknown DNA variants to be quickly identified, as well as providing an objective assessment of quality
for the DNA sequence generated. This chapter describes the Applied Biosystems SeqScape® software program (version 2.5) and how it has assisted in the interpretation of DNA sequencing in a DNA diagnostic laboratory.
Affiliation(s): (3) Department of Molecular and Clinical Genetics, Royal Prince Alfred Hospital, Camperdown, New South Wales, Australia
(4) Department of Molecular and Clinical Genetics, University of Sydney Central Clinical School, Royal Prince Alfred Hospital, Camperdown, New South Wales, Australia
(4) Department of Molecular and Clinical Genetics, University of Sydney Central Clinical School, Royal Prince Alfred Hospital, Camperdown, New South Wales, Australia
Book Title: Clinical Bioinformatics
Series: Methods in Molecular Medicine | Volume: 141 | Pub. Date: Dec-21-2007 | Page Range: 177-197 | DOI: 10.1007/978-1-60327-148-6_10
Subject: Bioinformatics
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