Springer Protocols: Abstract: Immunochemical Methods for the Rapid Screening of the O-Glycosidically Linked N-Acetylglucosamine Modification of Proteins

Immunochemical Methods for the Rapid Screening of the O-Glycosidically Linked N-Acetylglucosamine Modification of Proteins

By: Michael Ahrend³, Angela Käberich³, Marie-Therese Fergen³, Brigitte Schmitz³

Abstract

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for the rapid screening of specific post-translational modifications antibody-based methods are very well suited and applicable without demanding expenditure. Here we describe the immunochemical detection of the O-glycosidically linked cytosolic N-acetylglucosamine modification of proteins, which has attracted increasing interest in the last years. Two different monoclonal antibodies were used in enzyme-linked immunosorbent assays (ELISA), Western blots of 1- and 2- dimension (1D and 2D) separated proteins and immunohistochemical analysis of tissue sections. Slight differences in the recognition of this post-translational epitope by the 2 antibodies are observed and will be discussed.

Affiliation(s): (3) Department of Biochemistry, Institute of Animal Sciences, University of Bonn, Bonn, Germany

Book Title: Post-translational Modifi cations of Proteins: Tools for Functional Proteomics

Series: Methods in Molecular Biology | Volume: 446 | Pub. Date: Apr-04-2008 | Page Range: 267-280 | DOI: 10.1007/978-1-60327-084-7_19

Subject: Protein Science

Key Words: O-GlcNAc modification of proteins - posttranslational modification - immunochemical screening methods - ELISA - 1D and 2D Western blots - immuno-histochemical analysis

References

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