Springer Protocols: Abstract: Isolation of Phosphoproteins

Isolation of Phosphoproteins

By: Lawrence G. Puente³, Lynn A. Megeney³

Abstract

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Phosphorylation is one of the most abundant post-translational modifications on protein and one that frequently has functional biological consequences. For this reason, screening protein samples for phosphorylations has become an important tool in biochemical research. Affinity purification by immunological or chemical reagents can be used to isolate phosphoproteins from other cellular materials.

Affiliation(s): (3) The Ottawa Hospital, Ottawa, Canada

Book Title: 2D PAGE: Sample Preparation and Fractionation

Series: Methods in Molecular Biology | Volume: 424 | Pub. Date: Jan-25-2008 | Page Range: 365-372 | DOI: 10.1007/978-1-60327-064-9_28

Subject: Genetics/Genomics

Key Words: Affinity purification - immunoprecipitation - protein phosphorylation

References

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