Springer Protocols: Abstract: Protein Fractionation by Preparative Electrophoresis

Protein Fractionation by Preparative Electrophoresis

By: Michael Fountoulakis³, Ploumisti Dimitraki⁴

Abstract

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Preparative electrophoresis is a protein fractionation approach useful for the enrichment of low-abundance gene products. Preparative electrophoresis is usually performed in the PrepCell apparatus. Proteins are separated according to their size in a cylindrical gel in the presence of an ionic detergent. The method is particularly efficient for the enrichment of low-molecular-mass gene products. Preparative electrophoresis can be followed by proteomic analysis, and the proteins eluted from the preparative gel can be separated by two-dimensional gel electrophoresis and identified by mass spectrometry.

Affiliation(s): (3) F. Hoffmann-La Roche Ltd, Basel, Switzerland
(4) Academy of Athens, Athens, Greece

Book Title: 2D PAGE: Sample Preparation and Fractionation

Series: Methods in Molecular Biology | Volume: 424 | Pub. Date: Jan-25-2008 | Page Range: 301-313 | DOI: 10.1007/978-1-60327-064-9_24

Subject: Genetics/Genomics

Key Words: Low-abundance proteins - low-molecular-mass proteins - preparative electrophoresis - protein enrichment - proteomics

References

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