Springer Protocols: Abstract: Prefractionation, Enrichment, Desalting and Depleting of Low Volume and Low Abundance Proteins and Peptides Using the MF10

Prefractionation, Enrichment, Desalting and Depleting of Low Volume and Low Abundance Proteins and Peptides Using the MF10

By: Valerie Wasinger³, Linda Ly³, Anna Fitzgerald⁴, Brad Walsh⁴

Abstract

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The success of proteomics relies heavily in the ability to characterize very diverse species of proteins. This diversity stems from a proteins physicochemical properties, its copy number and abundance and its association with other proteins. Prefractionation and simplification of biological samples prior to downstream MS analysis is showing some virtue in obtaining greater depth of protein analysis. The MicroFlow MF10 is a prefractionation device for low volume, low abundance complex samples that can also enrich for very specific species of proteins based on charge and/or size either in native or denaturing format. It has also been used to desalt and deplete samples of contaminating ions or proteins. Although this instrument is only in its infancy in terms of exploring its capabilities, the technology has been used successfully for the fractionation of plasma proteins Wasinger(1), Omenn GS(2), as well as purification of human growth hormone Catzel D(3) antibodies Cheung(4) and IgY Gee(5) and other complex samples.

Affiliation(s): (3) University of NSW, Kensington, Australia
(4) Minomic, Chatswood West, Australia

Book Title: 2D PAGE: Sample Preparation and Fractionation

Series: Methods in Molecular Biology | Volume: 424 | Pub. Date: Jan-25-2008 | Page Range: 257-275 | DOI: 10.1007/978-1-60327-064-9_21

Subject: Genetics/Genomics

Key Words: Abundant protein removal - albumin - depletion - desalting - concentrating - electrophoresis - prefractionation - proteomic

References

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