Springer Protocols: Abstract: Bacteria and Yeast Cell Disruption Using Lytic Enzymes

Bacteria and Yeast Cell Disruption Using Lytic Enzymes

By: Oriana Salazar³

Abstract

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Enzymatic methods provide a convenient alternative for overcoming technical disadvantages of mechanical disruption. Protocols for protein extraction from bacteria and Saccharomyces cerevisiae using lytic enzymes are presented in this chapter. Adaptation of the yeast protocol to a microtiter plate format makes this protocol amenable for proteomic applications and high-throughput screening of libraries expressing genetic variants in yeast. This methodology can also be applied to bacteria.

Affiliation(s): (3) University of Chile, Santiago, Chile

Book Title: 2D PAGE: Sample Preparation and Fractionation

Series: Methods in Molecular Biology | Volume: 424 | Pub. Date: Jan-25-2008 | Page Range: 23-34 | DOI: 10.1007/978-1-60327-064-9_2

Subject: Genetics/Genomics

Key Words: Cell lysis - yeast-lytic endoglucanase - bacteriolytic enzyme - lysozyme - microtiter-plate format

References

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