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Enriching Basic and Acidic Rat Brain Proteins with Ion
Exchange Mini Spin Columns Before Two-Dimensional
Gel Electrophoresis
By: Ning Liu3 
, Aran Paulus3
, Aran Paulus3| Abstract |
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Proteome analysis by two-dimensional gel electrophoresis (2-DGE) faces significant challenges because of the complexity of
biological samples. However, the complexity of a protein sample can be reduced prior to 2-DGE by applying protein fractionation.
Protein fractionation allows analysis of one protein subset at a time, thereby, increasing the load of proteins of interest,
enriching low-abundance proteins, and increasing the resolution of protein spots on a 2-D gel.
Here we describe an ion exchange chromatography based method–-the use of anion or cation exchange (AEX or CEX) mini spin columns–-for
sample fractionation. Using rat brain tissues, we demonstrate that these mini spin columns provide an easy, convenient, and
reproducible way of fractionating brain proteins to enrich basic or acidic proteins before 2-DGE.
Book Title: 2D PAGE: Sample Preparation and Fractionation
Series: Methods in Molecular Biology | Volume: 424 | Pub. Date: Jan-25-2008 | Page Range: 157-166 | DOI: 10.1007/978-1-60327-064-9_14
Subject: Genetics/Genomics
Key Words: Acidic proteins - basic proteins - 2-D gel electrophoresis - ion exchange chromatography - mini spin column - rat brain - sample fractionation
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