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Radiolabeling for Two-Dimensional Gel Analysis

By: Hélian Boucherie³, Aurélie Massoni³, Christelle Monribot-Espagne⁴

Abstract

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Radiolabeling is a highly sensitive method for protein detection, which is easily performed by the incorporation of radioactive amino acids into proteins. This makes radiolabeling a method of choice for visualizing proteins separated on two-dimensional (2-D) gels. This chapter presents protocols to determine in vivo labeling conditions and to label proteins for the comparison of protein samples by means of 2-D gel electrophoresis.

Affiliation(s): (3) Centre National de la Recherche Scientifique, Bordeaux, France
(4) Centre National de la Recherche Scientifique, Gif-sur-Yvette, France

Book Title: 2D PAGE: Sample Preparation and Fractionation

Series: Methods in Molecular Biology | Volume: 424 | Pub. Date: Jan-25-2008 | Page Range: 125-135 | DOI: 10.1007/978-1-60327-064-9_11

Subject: Genetics/Genomics

Key Words: [35S]-Methionine labeling - protein synthesis - proteome analysis - radiolabeling - two-dimensional gel electrophoresis

References

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