SpringerProtocols: Abstract: Culture, Isolation, and Labeling of Anaplasma phagocytophilum for Subsequent Infection of Human Neutrophils

Culture, Isolation, and Labeling of Anaplasma phagocytophilum for Subsequent Infection of Human Neutrophils

By: Dori L. Borjesson³

Abstract

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Anaplasma phagocytophilum is the etiologic agent of granulocytic anaplasmosis, a tick-borne, zoonotic, emerging infectious disease. A. phagocytophilum is an obligate intracellular pathogen that primarily resides within membrane-bound, cytoplasmic vacuoles of host neutrophils. Closely related to Ehrlichial and Rickettsial organisms, A. phagocytophilum is a small, fragile, Gram-negative bacterium that presents unique challenges for culture, isolation, enumeration, and labeling. This chapter delineates pathogen-specific considerations for culture and labeling of this organism for subsequent use in assays to examine mechanisms of host cell–pathogen interactions.

Affiliation(s): (3) Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA

Book Title: Bacterial Pathogenesis: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 431 | Pub. Date: Feb-01-2008 | Page Range: 159-171 | DOI: 10.1007/978-1-60327-032-8_13

Subject: Microbiology

Key Words: Anaplasma phagocytophilum - granulocytic anaplasmosis - cell culture - fluorescent labeling - neutrophils

References

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