Phenolics are structurally assorted and are generally part of a complex mixture isolated from plant and biological origin matrices. A wide gamut of natural products have been the focus of main study for phenolic compounds while urine and blood are the two main biological fluids that have been analyzed for metabolism studies. Traditional and more advanced techniques have come to prominence for sample preparation, detection, and identification. This review is devoted to a short discussion of the occurrence of phenolic acids and flavonoids, their role in human health, and focuses on a detailed presentation of the analytical methods, concluding with the advantages of analytical methods employed so far and prospects. Strategies and practical aspects for the determination of phenolic acids and flavonoids in biological fluids, beverages, plants, and food are reported. Novel and past applications are provided with significant treatment and detection-related developments on the basis of the employment of separation and non-separation analytical techniques.
Affiliation(s): (1) Laboratory of Analytical Chemistry, Department of Chemistry, University of Ioannina, Ioannina, 451 10, Greece
Book Title: Free Radicals and Antioxidant Protocols
Series: Methods in Molecular Biology | Volume: 610 | Pub. Date: Sep-01-2008 | Page Range: 65-90 | DOI: 10.1007/978-1-60327-029-8_5
Copyright © 2008 Springer Science+Business Media, LLC. All rights reserved
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