SpringerProtocols: Abstract: A Method to Discover Phased siRNA Loci

5. A Method to Discover Phased siRNA Loci

By: Michael J. Axtell¹

Abstract

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Short, interfering RNAs (siRNAs) arise from the processing of long double-stranded RNA (dsRNA) by Dicer enzymes. Dicers generate siRNA duplexes by successive hydrolysis of both strands of the dsRNA phosphodiester backbone at positions determined by measuring 21–24 nucleotides from an exposed dsRNA terminus. Therefore, a population of dsRNAs with precisely identical termini will produce siRNA spaced in regular, 21–24-nucleotide intervals. This chapter presents an easily customized and generally applicable strategy for identifying loci which produce the “phased” siRNAs diagnostic of such processing. Given the input of a large set of expressed small RNAs and of the corresponding genome or transcriptome from which the small RNAs are derived, the methodology produces a ranking of user-defined loci with respect to their likely production of phased siRNAs. Top ranked loci are candidates for further computational and biological analyses.

Affiliation(s): (1) Department of Biology, Pennsylvania State University, University Park, PA, USA

Book Title: Plant MicroRNAs: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 592 | Pub. Date: Jan-01-2009 | Page Range: 59-70 | DOI: 10.1007/978-1-60327-005-2_5

Subject: Plant Sciences

Key Words: siRNA - microRNA - Trans-acting siRNA - Transitivity - Small RNA - Plants - Bioinformatics

References

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