SpringerProtocols: Abstract: Prediction of Plant miRNA Genes

2. Prediction of Plant miRNA Genes

By: Matthew W. Jones-Rhoades¹

Abstract

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This chapter presents procedures for the computational identification of plant miRNA genes. In the first procedure, homologs of known miRNAs are identified in a database of genomic or cDNA sequence. In the second procedure, previously unidentified miRNA families are predicted through the analysis of secondary structure, evolutionary conservation, and targeting potential.

Affiliation(s): (1) Department of Biology, Knox College, Galesburg, IL, USA

Book Title: Plant MicroRNAs: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 592 | Pub. Date: Jan-01-2009 | Page Range: 19-30 | DOI: 10.1007/978-1-60327-005-2_2

Subject: Plant Sciences

Key Words: Gene prediction - microRNA discovery - Comparative genomics

References

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