SpringerProtocols: Abstract: Purification of Proteins Using Displacement Chromatography

6. Purification of Proteins Using Displacement Chromatography

By: Nihal Tugcu²

Abstract

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Displacement chromatography has several advantages over the nonlinear elution technique, as well as the linear elution mode, such as the recovery of purified components at high concentrations, less tailing during elution, high throughput and high resolution. Displacer affinity and its utilization are the critical components of displacement chromatography. Particularly, the nonspecific interactions between the displacer and the stationary phase can be exploited to generate high affinity displacers. This chapter will discuss the design and execution of displacer selection and implementation in a separation specifically focusing on its utilization in ion exchange chromatography.

Affiliation(s): (2) Bioprocess R&D, BioPurification Development, Merck, Rahway, NJ

Book Title: Affinity Chromatography: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 421 | Pub. Date: Nov-30-2007 | Page Range: 71-90 | DOI: 10.1007/978-1-59745-582-4_6

Subject: Cell Biology

Key Words: Displacement - chromatography - protein purification - steric mass action isotherm

References

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