SpringerProtocols: Abstract: Affinity Precipitation of Proteins Using Metal Chelates

3. Affinity Precipitation of Proteins Using Metal Chelates

By: Ashok Kumar², Igor Yu. Galaev³, Bo Mattiasson⁴

Abstract

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Metal affinity precipitation has been successfully developed as a simple purification process for the proteins that have affinity for the metal ions. The copolymers of vinylimidazole with N-isopropylacrylamide are easily synthesized by radical polymerization. When loaded with Cu(II) and Ni(II) ions, these copolymers are capable of selectively precipitating proteins with natural metal-binding groups or histidine-tagged recombinant proteins.

Affiliation(s): (2) Department of Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur (IITK), Kanpur, India
(3) Department of Biotechnology, Centre for Chemistry and Chemical Engineering, Lund University, Lund, Sweden
(4) Centre for Chemistry and Chemical Engineering, Lund University, Lund, Sweden

Book Title: Affinity Chromatography: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 421 | Pub. Date: Nov-30-2007 | Page Range: 37-52 | DOI: 10.1007/978-1-59745-582-4_3

Subject: Cell Biology

Key Words: Metal chelate affinity precipitation - thermoresponsive copolymers - affinity macroligands - thermoprecipitation - bioseparation - recombinant histidine-tagged proteins

References

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