SpringerProtocols: Abstract: 4. Construction of Radiation Hybrid Panels

4. Construction of Radiation Hybrid Panels

By: John E. Page³, William J. Murphy⁴

Abstract

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Whole-genome radiation hybrid (RH) mapping has proven to be a powerful tool for mapping genes and comparing genome architecture. We describe a protocol for constructing RH panels by rescuing irradiated fibroblast donor cells of any mammalian species by polyethylene glycol fusion to a thymidine kinase-deficient hamster cell line. Characterization and expansion of a panel of 90–100 cell lines can be used to map virtually any PCR-based marker that can be distinguished from the recipient hamster genome. The described procedure has been used successfully to create RH panels from diverse mammalian species such as macaques, elephants, alpacas, and armadillos, and may be applicable to nonmammalian vertebrates as well.

Affiliation(s): (3) Integrated Toxicology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD
(4) Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX

Book Title: Phylogenomics

Series: Methods in Molecular Biology | Volume: 422 | Pub. Date: Dec-16-2007 | Page Range: 51-64 | DOI: 10.1007/978-1-59745-581-7_4

Subject: Cell Biology

Key Words: Comparative genomics - phylogenomics - radiation hybrid mapping - comparative gene mapping - A23 - HAT selection

References

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