SpringerProtocols: Abstract: 3. FISH for Mapping Single Copy Genes

3. FISH for Mapping Single Copy Genes

By: Terje Raudsepp³, Bhanu P. Chowdhary³

Abstract

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During the past two decades fluorescent in-situ hybridization (FISH) has become a standard technique to directly localize, orient, and order genes in the genomes of a wide range of species. Despite the availability of a variety of probes, probe labeling and signal-detection systems, and advanced image analysis software, the core procedures used to carry out FISH remain the same. A detailed overview of these procedures, including target preparation (metaphase/interphase chromosomes and DNA fibers), probe labeling, in-situ hybridization, signal detection, and imaging, is here provided in a stepwise manner.

Affiliation(s): (3) Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX

Book Title: Phylogenomics

Series: Methods in Molecular Biology | Volume: 422 | Pub. Date: Dec-16-2007 | Page Range: 31-49 | DOI: 10.1007/978-1-59745-581-7_3

Subject: Cell Biology

Key Words: FISH - gene mapping - metaphase chromosomes - interphase chromosomes - DNA fibers - DNA labeling

References

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