Springer Protocols: Abstract: Fluorescent Proteins for Cell Biology

Fluorescent Proteins for Cell Biology

By: George H. Patterson²

Abstract

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Through the use of exogenous labels, such as antibodies and synthetic fluorophores, experimenters have been able to readily observe the localization of proteins and organelles within a cell by fluorescence microscopy. The discovery and application of fluorescent proteins spanning a large wavelength range have revolutionized these studies. This chapter attempts to introduce the vast array of these molecules, discuss their characteristics, and assess the advantages and disadvantages that each displays for use in imaging.

Affiliation(s): (2) Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, USA

Book Title: Reporter Genes: A Practical Guide

Series: Methods in Molecular Biology | Volume: 411 | Pub. Date: Sep-13-2007 | Page Range: 47-80 | DOI: 10.1007/978-1-59745-549-7_5

Subject: Genetics/Genomics

Key Words: GFP - DsRed - photoactivatation - imaging

References

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