SpringerProtocols: Abstract: Localizing Proteins by Tissue Printing

10. Localizing Proteins by Tissue Printing

By: Rafael F. Pont-Lezica²

Abstract

Full Text

Download PDF (352K)

The simple technique of making tissue prints on appropriate substrate material has made possible the easy localization of proteins, nucleic acids, carbohydrates, and small molecules in a tissue-specific mode. Plant tissues can be used to produce prints revealing a remarkable amount of anatomical detail, even without staining, which might be used to record developmental changes over time. In this chapter we will focus on the protocols for the localization of proteins and glycans using antibodies or lectins, probably the most frequently used application, but the localization of other molecules is reported and the sources indicated.

Affiliation(s): (2) Surfaces Cellulaires et Signalisation chez les Végétaux, UMR 5546 CNRS-Université Paul Sabatier-Toulouse III, Pôle de Biotechnologie végétale, 24 Chemin de Borde Rouge BP, 42617 Auzeville, 31326 Castanet-Tolosan, France

Book Title: Protein Blotting and Detection: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 536 | Pub. Date: Feb-06-2009 | Page Range: 75-88 | DOI: 10.1007/978-1-59745-542-8_10

Subject: Protein Science

Key Words: Immunodetection - Tissue blotting - Protein localization - Western blotting

References

Download References

1.	Daoust, R. (1957) Localization of deoxyribonuclease in tissue sections. A new approach to the histochemistry of enzymes. Exp Cell Res. 12, 203–211.

2.Reid, P.D., Pont-Lezica, R.F., del Campillo, E., and Taylor, R. (eds.) (1992) Tissue Printing. Academic Press, San Diego, CA.

3.	Varner, J. and Ye, Z. (1994) Tissue printing. FASEB J. 8, 378–384.

4.	Tieman, D.M. and Handa, A. (1989) Immunocytolocalization of polugalacturonase in ripening tomato fruit. Plant Physiol. 90, 17–20.

5.	Pont-Lezica, R.F., Taylor, R., and Varner, J. (1991) Solanum tuberosum aggultinin accumulation during tuber development. J Plant Physiol. 137, 453–458.

6.	Jacobsen, J. and Knox, R. (1973) Cytochemical localization and antigenicity of α-amylase in barley aleurone tissue. Planta 112, 213–224.

7.	Nolan, M. and David, B. (1984) A starch-agar gel method for the localization of starch hydrolyzing enzymes in the cotyledons and hypocotyls of beans. Am J Bot. 71, 137–141.

8.	Spruce, J., Mayer, A.M., and Osborne, D.J. (1987) A simple histochemical method for locating enzymes in plant tissue using nitrocellulose blotting. Phytochemistry 26, 2901–2903.

9.	Pearse, A.G.E. (1980) Substrate film techniques, In: Histochemistry, Theoretical and Applied, Churchill Livingstone, Edinburgh, pp. 253–269.

10.	Roth, R., Boudet, A., and Pont-Lezica, R.F. (1997) Lignification and cinnamyl alcohol dehydrogenase activity in developing stems of tomato and polar: a spatial and kinetic study through tissue printing. J Exp Bot. 48, 247–254.

11.	da Silva, E., Toorop, P., van Aelst, A., and Hilhorst, H. (2004) Abscisic acid controls embryo growth potential and endosperm cap weakening during coffee (Cofea arabica cv. Rubi) seed germination. Planta. 220, 251–261.

12.	Driouich, A., Lainé, A., Vian, B., and Faye, L. (1992) Characterization and localization of laccase forms in stem and cell cultures of sycamore. Plant J. 2, 13–24.

13.	Hara, M., Eto, H., and Kuboi, T. (2001) Tissue printing for myrosinase activity in roots of turnip and japanese radish and horseradish: a technique for localizing myrosinases. Plant Sci. 160, 425–431.

14.	Cassab, G.I., Lin, J.J., Lin, L.S., and Varner, J.E. (1988) Ethylene effect on extensin and peroxidase distribution in the subapical region of pea epicotyls. Plant Physiol. 88, 522–524.

15.	Maki, H. and Morohashi, Y. (2006) Development of polyphenol oxidase activity in the micropylar endosperm of tomato seeds. J Plant Physiol. 163, 1–10.

16.	Yomo, H. and Taylor, M.P. (1973) Histochemical studies on protease formation in the cotyledons of germinating bean seeds. Planta 112, 35–43.

17.	Harris, N. and Chrispeels, M.J. (1975) Histochemical and biochemical observations on storage protein metabolism and protein body autolysis in cotyledons of germinating beans. Plant Physiol. 56, 292–299.

18.	Moore, B., Kang, B., and Flurkey, W. (1989) Histochemical localization of mushroom tyrosinase in whole sections on nitrocellulose. Histochemistry 90, 379–381.

19.	Singh, M.B. and Knox, R. (1985) Grass pollen allergens: antigenic relationships detected using monoclonal antibodies and dot immunobinding assay. Int Arch Allergy Appl Immunol 78, 300–304.

20.	Antikainen, M., Griffith, M., Zhang, J., Hon, W., Yang, D., and Pihakaski-Maunsbach, K. (1996) Immunolocalization of antifreeze proteins in winter rye leaves, crowns, and roots by tissue printing. Plant Physiol. 110, 845–857.

21.	Ramalho-Santos, M., Pissarra, J., Verissimo, P., Pereira, S., Salema, R., Pires, E., et al (1997) Cardosin a, an abundant aspartic proteinase, accumulates in protein storage vacuoles in the stigmatic papillae of Cynara cardunculus L. Planta. 203, 204–212.

22.	del Campillo, E., Reid, P., Sexton, R., and Lewis, L. (1990) Occurrence and localization of 9.5 cellulase in abscising and nonabscising tissues. Plant Cell 2, 245–254.

23.	Reid, P.D., del Campillo, E., and Lewis, L.N. (1990) Anatomical changes and immunolocalization of cellulase during abscission as observed on nitrocellulose tissue prints. Plant Physiol. 93, 160–165.

24.	Pont-Lezica, R.F. and Varner, J.E. (1989) Histochemical localization of cysteine-rich proteins by tissue printing on nitrocellulose. Anal Biochem. 182, 334–337.

25.	Bailey, B.A., Taylor, R., Dean, J.F., and Anderson, J.D. (1991) Ethylene biosynthesis-inducing endoxylanase is translocated through the xylem of Nicotiana tabacum cv xanthi plants. Plant Physiol. 97, 1181–1186.

26.	Cho, H. and Kende, H. (1997) Expansins and internodal growth of deepwater rice. Plant Physiol. 113, 1145–1151.

27.	Cassab, G.I. and Varner, J.E. (1987) Immunocytolocalization of extensin in developing soybean seed coats by immunogold-silver staining and by tissue printing on nitrocellulose paper. J Cell Biol. 105, 2581–2588.

28.	Hood, K., Baasiri, R., Fritz, S., and Hood, E. (1991) Biochemical and tissue print analyses of hydroxyproline-rich glycoproteins in cell walls of sporophytic maize tissues. Plant Physiol. 96, 1214–1219.

29.	Ye, Z.H., Song, Y.R., Marcus, A., and Varner, J.E. (1991) Comparative localization of three classes of cell wall proteins. Plant J. 1, 175–183.

30.	Goto, F., Yoshihara, T., Shigemoto, N., Toki, S., and Takaiwa, F. (1999) Iron fortification of rice seed by the soybean ferritin gene. Nature Biotechnol. 17, 282–286.

31.	Pereira, S., Carvalho, H., Sunkel, C., and Salema, R. (1992) Immunocytolocalization of glutamine synthase in mesophyll and phloem of leaves of Solanum tuberosum L. Protoplasma. 167, 66–73.

32.	Keller, B., Sauer, N., and Lamb, C.J. (1988) Glycine-rich cell wall proteins in bean: gene structure and association of the proteins with the vascular system. EMBO J. 7, 3625–3633.

33.	Ye, Z.H., and Varner, J. (1991) Tissue-specific expression of cell wall proteins in developing soybean tissues. Plant Cell. 3, 23–37.

34.	Ryser, U. and Keller, B. (1992) Ultrastructural localization of a bean glycine-rich protein in unlignified primary walls of protoxylem cells. Plant Cell 4, 773–783.

35.	Gabius, S., Hellmann, K.P., Hellmann, T., Brinck, U., and Gabius, H.J. (1989) Neoglycoenzymes: a versatile tool for lectin detection in solid phase assays and glycohistochemistry. Anal Biochem. 182, 447–451.

36.	Gonorazky, A., Regente, M., and de la Canal, L. (2005) Stress induction and antimicrobial properties of a lipid transfer protein in germinating sunflower seeds. J Plant Physiol. 162, 618–624.

37.	Kausch, K. and Handa, A. (1997) Molecular cloning of a ripening-specific lipoxygenase and its expression during wild-type and mutant tomato fruit development. Plant Physiol. 113, 1041–1050.

38.	Maryani, M., Morse, M., Bradley, G., Irving, H., Cahill, D., and Gehring, C. (2003) In situ localization associates biologically active plant natriuretic peptide immuno-analogues with conductive tissue and stomata. J Exp Bot. 54, 1553–1564.

39.	Fujita, Y., Fujita, M., Mise, K., Kobori, T., Osaki, T., and Furusawa, I. (2000) Bromovirus movement protein conditions for the host specificity of virus movement through the vascular system and affects pathogenicity in cowpea. Mol Plant Microbe Interact. 13, 1195–1203.

40.	Dicenta, F., Martinez-Gomez, P., Bellanger, I., and Audergon, J. (2000) Localization of plum pox virus in stem and petiole tissues of apricot cultivars by immuno-tissue printing. Acta Virol. 44, 323–328.

41.	Knapp, E., da Camara Machado, A., Puhringer, H., Wang, Q., Hanzer, V., Weiss, H., et al (1995) Localization of fruit tree viruses by immuno-tissue printing in infected shoots of Malus sp. and Prunus sp. J Virol Methods. 55, 157–173.

42.	Mansky, L.M., Andrews, R.E., Durand, D.P., and Hill, J.H. (1990) Plant virus localization in leaf tissue by press blotting. Plant Mol Biol Rep. 8, 13–17.

43.	Holt, C.A. and Beachy, R.N. (1991) In vivo complementation of infectious transcripts from mutant tobacco mosaic virus cDNAs in transgenic plants. Virology 181, 109–117.

44.	Jung, G.L. and Hahne, G. (1992) A simple method to increase resolution in whole leaf blotting. Plant Sci. 82, 125–132.

45.	Lin, N.S., Hsu, Y.H., and Hsu, H.T. (1990) Immunological detection of plant viruses and mycoplasma-like organism by direct tissue blotting on nitrocellulose membranes. Phytopathology. 80, 824–828.

46.	Polston, J.E., Bubrick, P., and Perring, T.M. (1991) Detection of plant virus coat proteins on whole leaf blots. Anal Biochem. 196, 267–270.

47.	Burlat, V., Kwon, M., Davin, L., and Lewis, N. (2001) Dirigent proteins and dirigent sites in lignifying tissues. Phytochemistry 57, 883–897.

48.	Willats, W., Marcus, S., and Knox, J. (1998) Generation of monoclonal antibody specific to (1–5)-á-l-arabinan. Carbohydr Res. 308, 149–152.

49.	Varner, J.E., Song, Y.R., Lin, L.S., and Yuen, H. (1989) The role f cell wall proteins in the structure and function of cell walls, In: The molecular Basis of Plant Development (Goldberg, R., ed.), Alan R Lis, New York, pp. 161–168.

50.	Harriman, R.W.F., Tieman, D.M., and Handa, A.K. (1992) Localization of pectin methylesterase mRNA in tomato fruit, In: Tissue Printing (Reid, P.D., et al., eds.), Ademic Press, San Diego, CA, pp. 121–123.

51.	McClure, B. and Guilfoyle, T. (1989) Tissue print hybridization. A simple technique for detecting organ- and tissue-specific gene expression. Plant Mol Biol. 12, 517–524.

52.	Pont-Lezica, R.F. (1992) Lectin and glycan recognition, In: Tissue Printing (Reid, P.D., et al., eds.), Academic Press, San Diego, CA, pp. 71–79.

53.	Jones, L., Seymour, G., and Knox, J. (1997) Localization of pectic galactan in tomato cell walls using a monoclonal antibody specific to (1,4)-â-d-galactan. Plant Physiol. 113, 1405–1412.

54.	Hancock, K. and Tsang, V.C.W. (1983) India ink staining for protein on nitrocellulose paper. Anal Biochem. 133, 157–162.

55.	Jamet, E., Canut, H., Boudart, G., and Pont-Lezica, R.F. (2006) Cell wall proteins: a new insight through proteomics. Trends Plant Sci. 11, 33–39.

Comments (Loading...)

Post comment/View all comments