SpringerProtocols: Abstract: Identification of Inhibitors of Chromatin Modifying Enzymes Using the Yeast Phenotypic Screens

Identification of Inhibitors of Chromatin Modifying Enzymes Using the Yeast Phenotypic Screens

By: Benjamin Newcomb², Antonio Bedalov²

Abstract

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A multitude of enzymes that modify histones and remodel nucleosomes are required for the formation, maintenance, and propagation of the transcriptionally repressed chromatin state in eukaryotes. Robust phenotypic screens in yeast S. cerevisiae have proved instrumental in identifying these activities and for providing mechanistic insights into epigenetic regulation. These phenotypic assays, amenable for high throughput small molecule screening, enable identification and characterization of inhibitors of chromatin modifying enzymes largely bypassing traditional biochemical approaches.

Affiliation(s): (2) Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue N, MS D2-100, P.O. Box 19024, Seattle, WA 98109, USA

Book Title: Yeast Functional Genomics and Proteomics: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 548 | Pub. Date: Jul-01-2009 | Page Range: 145-160 | DOI: 10.1007/978-1-59745-540-4_8

Subject: Protein Science

Key Words: Chromatin - Chemical genetics - Histone deacetylase - Chromatin modifying enzyme - Epigenetics

References

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