SpringerProtocols: Abstract: A Genomic Approach to Yeast Chronological Aging

A Genomic Approach to Yeast Chronological Aging

By: Christopher R. Burtner², Christopher J. Murakami², Matt Kaeberlein²

Abstract

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Yeast is a useful model organism to study the genetic and biochemical mechanisms of aging. Genomic studies of aging in yeast have been limited, however, by traditional methodologies that require a large investment of labor and resources. In this chapter, we describe a newly-developed method for quantitatively measuring the chronological life span of each strain contained in the yeast ORF deletion collection. Our approach involves determining population survival by monitoring outgrowth kinetics using a Bioscreen C MBR shaker/incubator/plate reader. This method has accuracy comparable to traditional assays, while allowing for higher throughput and decreased variability in measurement.

Affiliation(s): (2) Department of Pathology, University of Washington, Box 357470 Seattle, WA 98195-7470, USA

Book Title: Yeast Functional Genomics and Proteomics: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 548 | Pub. Date: Jul-01-2009 | Page Range: 101-114 | DOI: 10.1007/978-1-59745-540-4_6

Subject: Protein Science

Key Words: Longevity - Aging - Chronological life span, - Yeast - Bioscreen - Stationary phase

References

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1.	Kaeberlein, M. (2006). In Handbook of models for human aging. (Conn, P. M., Ed.), Elsevier Press, Boston, pp. 109–120.

2.	Mortimer, R. K., and Johnston, J. R. (1959). Life span of individual yeast cells. Nature. 183, 1751–1752.

3.	Fabrizio, P., and Longo, V. D. (2003). The chronological life span of Saccharomyces cerevisiae. Aging Cell. 2, 73–81.

4.	Kaeberlein, M. (2006). Genome-wide approaches to understanding human ageing. Hum Genomics. 2, 422–428.

5.	Jiang, J. C., Jaruga, E., Repnevskaya, M. V., and Jazwinski, S. M. (2000). An intervention resembling caloric restriction prolongs life span and retards aging in yeast. Faseb J. 14, 2135–2137.

6.	Kaeberlein, M., Kirkland, K. T., Fields, S., and Kennedy, B. K. (2004). Sir2-independent life span extension by calorie restriction in yeast. PLoS Biol. 2, E296.

7.	Lin, S. J., Defossez, P. A., and Guarente, L. (2000). Requirement of NAD and SIR2 for life-span extension by calorie restriction in Saccharomyces cerevisiae. Science. 289, 2126–2128.

8.	Fabrizio, P., Gattazzo, C., Battistella, L., Wei, M., Cheng, C., McGrew, K., and Longo, V. D. (2005). Sir2 blocks extreme life-span extension. Cell. 123, 655–667.

9.	Smith Jr, D. L., McClure, J. M., Matecic, M., and Smith, J. S. (2007). Calorie restriction extends the chronological lifespan of Saccharomyces cerevisiae independently of the Sirtuins. Aging Cell. 6, 649–62.

10.	Powers, R. W., 3rd, Kaeberlein, M., Caldwell, S. D., Kennedy, B. K., and Fields, S. (2006). Extension of chronological life span in yeast by decreased TOR pathway signaling. Genes Dev. 20, 174–184.

11.	Kaeberlein, M., Powers, R. W., 3rd, Steffen, K. K., Westman, E. A., Hu, D., Dang, N., Kerr, E. O., Kirkland, K. T., Fields, S., and Kennedy, B. K. (2005). Regulation of yeast replicative life span by TOR and Sch9 in response to nutrients. Science. 310, 1193–1196.

12.	Kaeberlein, M., McVey, M., and Guarente, L. (1999). The SIR2/3/4 complex and SIR2 alone promote longevity in Saccharomyces cerevisiae by two different mechanisms. Genes Dev. 13, 2570–2580.

13.	Fabrizio, P., Liou, L. L., Moy, V. N., Diaspro, A., SelverstoneValentine, J., Gralla, E. B., and Longo, V. D. (2003). SOD2 functions downstream of Sch9 to extend longevity in yeast. Genetics. 163, 35–46.

14.	Fabrizio, P., Pletcher, S. D., Minois, N., Vaupel, J. W., and Longo, V. D. (2004). Chronological aging-independent replicative life span regulation by Msn2/Msn4 and Sod2 in Saccharomyces cerevisiae. FEBS Lett. 557, 136–142.

15.	Fabrizio, P., Pozza, F., Pletcher, S. D., Gendron, C. M., and Longo, V. D. (2001). Regulation of longevity and stress resistance by Sch9 in yeast. Science. 292, 288–290.

16.	Kaeberlein, M., Burtner, C. R., and Kennedy, B. K. (2007). Recent developments in yeast aging. PLoS Genet. 3, e84.

17.	Kaeberlein, M., and Kennedy, B. K. (2005). Large-scale identification in yeast of conserved ageing genes. Mech Ageing Dev. 126, 17–21.

18.	Malathi, K., Higaki, K., Tinkelenberg, A. H., Balderes, D. A., Almanzar-Paramio, D., Wilcox, L. J., Erdeniz, N., Redican, F., Padamsee, M., Liu, Y., Khan, S., Alcantara, F., Carstea, E. D., Morris, J. A., and Sturley, S. L. (2004). Mutagenesis of the putative sterol-sensing domain of yeast Niemann Pick C-related protein reveals a primordial role in subcellular sphingolipid distribution. J Cell Biol. 164, 547–556.

19.	Tsuchiya, M., Dang, N., Kerr, E. O., Hu, D., Steffen, K. K., Oakes, J. A., Kennedy, B. K., and Kaeberlein, M. (2006). Sirtuin-independent effects of nicotinamide on lifespan extension from calorie restriction in yeast. Aging Cell. 5, 505–514.

20.	Murakami, C. J., Burtner, C. R., Kennedy, B. K., and Kaeberlein, M. (2008). A method for high-throughput quantitative analysis of yeast chronological life span. J Gerontol A Biol Sci Med Sci. 63, 113–21.

21.	Winzeler, E. A., Shoemaker, D. D., Astromoff, A., Liang, H., Anderson, K., Andre, B., Bangham, R., Benito, R., Boeke, J. D., Bussey, H., Chu, A. M., Connelly, C., Davis, K., Dietrich, F., Dow, S. W., El Bakkoury, M., Foury, F., Friend, S. H., Gentalen, E., Giaever, G., Hegemann, J. H., Jones, T., Laub, M., Liao, H., Davis, R. W., and et al (1999). Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science. 285, 901–906.

22.	Fabrizio, P., Battistella, L., Vardavas, R., Gattazzo, C., Liou, L. L., Diaspro, A., Dossen, J. W., Gralla, E. B., and Longo, V. D. (2004). Superoxide is a mediator of an altruistic aging program in Saccharomyces cerevisiae. J Cell Biol. 166, 1055–1067.

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