Springer Protocols: Abstract: Generation of a Monoclonal Antibody Library Against Human Embryonic Stem Cells

Generation of a Monoclonal Antibody Library Against Human Embryonic Stem Cells

By: Micha Drukker³, Christina Muscat³, Irving L. Weissman³

Abstract

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Differentiated cell types derived from human embryonic stem cells (hESCs) may serve in the future to treat various human diseases and to model early human embryonic development in vitro. Fulfilling this potential, however, requires extensive development of methods and reagents for studying hESCs self-renewal and differentiation. One of the most widely used experimental approaches in the field of stem cell research is the identification of cell surface markers that can be used to prospectively define and isolate specific populations of stem cells and their progenitors. Here, we review an efficient method for generating monoclonal antibodies against cell surface antigens expressed by hESCs and stem cells at different stages of differentiation. This method may have profound implications for many aspects of hESC research and therapeutics.

Affiliation(s): (3) Department of Pathology, Stanford University School of Medicine, Stanford, CA

Book Title: Stem Cell Assays

Series: Methods in Molecular Biology | Volume: 407 | Pub. Date: Aug-10-2007 | Page Range: 63-81 | DOI: 10.1007/978-1-59745-536-7_6

Subject: Cell Biology

Key Words: Human embryonic stem cells - Monoclonal antibodies

References

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1.	Thomson, J. A., Itskovitz-Eldor, J., Shapiro, S. S., Waknitz, M. A., Swiergiel, J. J., Marshall, V. S., et al. (1998) Embryonic stem cell lines derived from human blastocysts. Science 282, 1145–1147.

2.	Reubinoff, B. E., Pera, M. F., Fong, C. Y., Trounson, A. and Bongso, A. (2000) Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro. Nat. Biotechnol. 18, 399–404.

3.	Itskovitz-Eldor, J., Schuldiner, M., Karsenti, D., Eden, A., Yanuka, O., Amit, M., et al. (2000) Differentiation of human embryonic stem cells into embryoid bodies comprising the three embryonic germ layers. Mol. Med. 6, 88–95.

4.	Carpenter, M. K., Inokuma, M. S., Denham, J., Mujtaba, T., Chiu, C. P. and Rao, M. S. (2001) Enrichment of neurons and neural precursors from human embryonic stem cells. Exp. Neurol. 172, 383–397.

5.	Reubinoff, B. E., Itsykson, P., Turetsky, T., Pera, M. F., Reinhartz, E., Itzik, A., et al. (2001) Neural progenitors from human embryonic stem cells. Nat. Biotechnol. 19, 1134–1140.

6.	Schuldiner, M., Eiges, R., Eden, A., Yanuka, O., Itskovitz-Eldor, J., Goldstein, R. S., et al. (2001) Induced neuronal differentiation of human embryonic stem cells. Brain Res. 913, 201–205.

7.	Zhang, S. C., Wernig, M., Duncan, I. D., Brustle, O. and Thomson, J. A. (2001) In vitro differentiation of transplantable neural precursors from human embryonic stem cells. Nat. Biotechnol. 19, 1129–1133.

8.	Schulz, T. C., Palmarini, G. M., Noggle, S. A., Weiler, D. A., Mitalipova, M. M. and Condie, B. G. (2003) Directed neuronal differentiation of human embryonic stem cells. BMC Neurosci. 4, 27.

9.	Kehat, I., Kenyagin-Karsenti, D., Snir, M., Segev, H., Amit, M., Gepstein, A., et al. (2001) Human embryonic stem cells can differentiate into myocytes with structural and functional properties of cardiomyocytes. J. Clin. Invest. 108, 407–414.

10.	Kehat, I., Gepstein, A., Spira, A., Itskovitz-Eldor, J. and Gepstein, L. (2002) High-resolution electrophysiological assessment of human embryonic stem cell-derived cardiomyocytes: a novel in vitro model for the study of conduction. Circ. Res. 91, 659–661.

11.	Mummery, C., Ward, D., van den Brink, C. E., Bird, S. D., Doevendans, P. A., Opthof, T., et al. (2002) Cardiomyocyte differentiation of mouse and human embryonic stem cells. J. Anat. 200, 233–242.

12.	Xu, C., Police, S., Rao, N. and Carpenter, M. K. (2002) Characterization and enrichment of cardiomyocytes derived from human embryonic stem cells. Circ. Res. 91, 501–508.

13.	Mummery, C., Ward-van Oostwaard, D., Doevendans, P., Spijker, R., van den Brink, S., Hassink, R., et al. (2003) Differentiation of human embryonic stem cells to cardiomyocytes: role of coculture with visceral endoderm-like cells. Circulation 107, 2733–2740.

14.	He, J. Q., Ma, Y., Lee, Y., Thomson, J. A. and Kamp, T. J. (2003) Human embryonic stem cells develop into multiple types of cardiac myocytes: action potential characterization. Circ. Res. 93, 32–39.

15.	Levenberg, S., Golub, J. S., Amit, M., Itskovitz-Eldor, J. and Langer, R. (2002) Endothelial cells derived from human embryonic stem cells. Proc. Natl. Acad. Sci. U. S. A. 99, 4391–4396.

16.	Kaufman, D. S., Hanson, E. T., Lewis, R. L., Auerbach, R. and Thomson, J. A. (2001) Hematopoietic colony-forming cells derived from human embryonic stem cells. Proc. Natl. Acad. Sci. U. S. A. 98, 10716–10721.

17.	Chadwick, K., Wang, L., Li, L., Menendez, P., Murdoch, B., Rouleau, A., et al. (2003) Cytokines and BMP-4 promote hematopoietic differentiation of human embryonic stem cells. Blood 102, 906–915.

18.	Green, H., Easley, K. and Iuchi, S. (2003) Marker succession during the development of keratinocytes from cultured human embryonic stem cells. Proc. Natl. Acad. Sci. U. S. A. 100, 15625–15630.

19.	Sottile, V., Thomson, A. and McWhir, J. (2003) In vitro osteogenic differentiation of human ES cells. Cloning Stem Cells 5, 149–155.

20.	Rambhatla, L., Chiu, C. P., Kundu, P., Peng, Y. and Carpenter, M. K. (2003) Generation of hepatocyte-like cells from human embryonic stem cells. Cell Transplant. 12, 1–11.

21.	Lavon, N., Yanuka, O. and Benvenisty, N. (2004) Differentiation and isolation of hepatic-like cells from human embryonic stem cells. Differentiation 72, 230–238.

22.	Schuldiner, M., Itskovitz-Eldor, J. and Benvenisty, N. (2003) Selective ablation of human embryonic stem cells expressing a “suicide” gene. Stem Cells 21, 257–265.

23.	Lavon, N., Yanuka, O. and Benvenisty, N. (2006) The effect of over expression of Pdx1 and Foxa2 on the differentiation of human embryonic stem cells into pancreatic cells. Stem Cells. 24, 1923–1930.

24.	Kohler, G. and Milstein, C. (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256, 495–497

25.	Yin, A. H., Miraglia, S., Zanjani, E. D., Almeida-Porada, G., Ogawa, M., Leary, A. G., et al. (1997) AC133, a novel marker for human hematopoietic stem and progenitor cells. Blood 90, 5002–5012.

26.	Weissman, I. L., Peacock, M. and Eltringham, J. R. (1973) Regional lymph node irradiation: effect on local and distant generation of antibody forming cells. J. Immunol. 110, 1300–1306.

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