Springer Protocols: Abstract: Digital Imaging of Stem Cells by Electron Microscopy

Digital Imaging of Stem Cells by Electron Microscopy

By: A. Henry Sathananthan³, Stefania A. Nottola⁴

Abstract

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This chapter deals with basic techniques of scanning and transmission electron microscopy applicable to stem cell imaging. It is sometimes desirable to characterize the fine structure of embryonic and adult stem cells to supplement the images obtained by phase-contrast and confocal immunofluorescent microscopy to compare with the microstructure of cells and tissues reported in the literature. This would help confirm their true identity whilst defining their surface and internal morphology. The intention is to put a face on stem cells during their differentiation.

Affiliation(s): (3) Monash Immunology & Stem Cell Laboratories, Monash University, Melbourne, Australia
(4) Department of Anatomy, Laboratory for Electron Microscopy “Pietro M. Motta”, La Sapienza University, Rome, Italy

Book Title: Stem Cell Assays

Series: Methods in Molecular Biology | Volume: 407 | Pub. Date: Aug-10-2007 | Page Range: 21-41 | DOI: 10.1007/978-1-59745-536-7_3

Subject: Cell Biology

Key Words: SEM - TEM - Stem cells - Embryonic - Adult - Microstructure - Imaging

References

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