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Genetic Analysis of Meiotic Recombination in Schizosaccharomyces pombe
Abstract
The fission yeast Schizosaccharomyces pombe is well-suited for studying meiotic recombination. Methods are described here for culturing S. pombe and for genetic assays of intragenic recombination (gene conversion), intergenic recombination (crossing-over), and spore viability. Both random spore and tetrad analyses are described.
Affiliation(s): (1) Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA, USA
Series: Methods in Molecular Biology  |  Volume: 557  |  Pub. Date: Aug-01-2009  |  Page Range: 65-76  |  DOI: 10.1007/978-1-59745-527-5_6
Subject:  Cell Biology
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