Springer Protocols: Abstract: Nuclear Magnetic Resonance Structural Studies of Membrane Proteins in Micelles and Bilayers

Nuclear Magnetic Resonance Structural Studies of Membrane Proteins in Micelles and Bilayers

By: Xiao-Min Gong², Carla M. Franzin², Khang Thai², Jinghua Yu², Francesca M. Marassi²

Abstract

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Nuclear magnetic resonance (NMR) spectroscopy enables determination of membrane protein structures in lipid environments, such as micelles and bilayers. This chapter outlines the steps for membrane-protein structure determination using solution NMR with micelle samples, and solid-state NMR with oriented lipid-bilayer samples. The methods for protein expression and purification, sample preparation, and NMR experiments are described and illustrated with examples from γ and CHIF, two membrane proteins that function as regulatory subunits of the Na⁺- and K⁺-ATPase.

Affiliation(s): (2) The Burnham Institute, La Jolla, CA, USA

Book Title: Methods in Membrane Lipids

Series: Methods in Molecular Biology | Volume: 400 | Pub. Date: Aug-30-2007 | Page Range: 515-529 | DOI: 10.1007/978-1-59745-519-0_35

Subject: Biochemistry

Key Words: Bilayer membrane - expression - FXYD - lipid - micelle - NMR - protein - structure

References

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