Springer Protocols: Abstract: Measurement of Lipid Forces by X-Ray Diffraction and Osmotic Stress

Measurement of Lipid Forces by X-Ray Diffraction and Osmotic Stress

By: Horia I. Petrache^{2 3}, Daniel Harries³, V. Adrian Parsegian³

Abstract

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Lipid suspensions in aqueous solutions most often form multilamellar vesicles of uniformly spaced bilayers. Interlamellar spacing is determined by the balance of attractive van der Waals (charge fluctuation) and repulsive forces. This balance of forces, as well as membrane elasticity, can be probed by applied osmotic stress. We describe how osmotic stress can be imposed on multilamellar lipid samples to study lipid interactions.

Affiliation(s): (2) Department of Physics, Indiana University-Purdue University, Indianapolis, IN, USA
(3) Laboratory of Physical and Structural Biology, NICHHD, National Institutes of Health, Bethesda, MD, USA

Book Title: Methods in Membrane Lipids

Series: Methods in Molecular Biology | Volume: 400 | Pub. Date: Aug-30-2007 | Page Range: 405-419 | DOI: 10.1007/978-1-59745-519-0_27

Subject: Biochemistry

Key Words: Bending rigidity - cholesterol hydration - membrane fluctuations - osmotic pressure - spontaneous curvature

References

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