Springer Protocols: Abstract: Fluorescence Microscopy to Study Pressure Between Lipids in Giant Unilamellar Vesicles

Fluorescence Microscopy to Study Pressure Between Lipids in Giant Unilamellar Vesicles

By: Anna Celli², Claudia Y. C. Lee², Enrico Gratton²

Abstract

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The authors developed a technique to apply high hydrostatic pressure to giant unilamellar vesicles and to directly observe the consequent structural changes with two-photon fluorescence microscopy imaging using high numerical aperture oil-immersion objectives. The data demonstrate that high pressure has a dramatic effect on the shape of the vesicles, and both fluidity and homogeneity of the membrane.

Affiliation(s): (2) Laboratory of Fluorescence Dynamics, Department of Physics, University of Illinois, Urbana-Champaign, IL, USA

Book Title: Methods in Membrane Lipids

Series: Methods in Molecular Biology | Volume: 400 | Pub. Date: Aug-30-2007 | Page Range: 333-339 | DOI: 10.1007/978-1-59745-519-0_22

Subject: Biochemistry

Key Words: Microscopy - pressure - vesicles

References

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