SpringerProtocols: Abstract: Protein Targeting to Mitochondria of Saccharomyces cerevisiae and Neurospora crassa: In Vitro and In Vivo Studies

Protein Targeting to Mitochondria of Saccharomyces cerevisiae and Neurospora crassa: In Vitro and In Vivo Studies

By: Panagiotis Papatheodorou², Grażyna Domańska², Joachim Rassow²

Abstract

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Most studies on the biogenesis of mitochondrial proteins have been carried out using fungal mitochondria as a model system. In particular, baker’s yeast, Saccharomyces cerevisiae, combines several experimental advantages, allowing both genetic and biochemical approaches and thus a combination of investigations in vivo and in vitro. However, the red bread mold Neurospora crassa has also been an important research tool. Isolated mitochondria can be used from both organisms for import experiments in a reconstituted system, using radiolabeled precursor proteins synthesized in reticulocyte lysate or purified preproteins. Assays are available for studies on the import pathways and localization of mitochondrial proteins and for the characterization of the components of the protein import machinery.

Affiliation(s): (2) Institut für Physiologische Chemie, Ruhr-Universität Bochum, Bochum, Germany

Book Title: Protein Targeting Protocols

Series: Methods in Molecular Biology | Volume: 390 | Pub. Date: May-21-2007 | Page Range: 151-166 | DOI: 10.1007/978-1-59745-466-7_10

Subject: Protein Science

Key Words: Mitochondria - yeast - in vitro assay - protein targeting - protein translocation.

References

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