SpringerProtocols: Abstract: Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus System

Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus System

By: James A. Fornwald², Quinn Lu³, Dayuan Wang³, Robert S. Ames³

Abstract

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BacMams are modified baculoviruses that contain mammalian expression cassettes for gene delivery and expression in mammalian cells. The BacMam system combines the advantages of viral transient expression, ease in generation, and a wide cell tropism. It enables rapid, facile, and flexible gene over-expression experiments to be performed in a variety of mammalian cell lines. Conversion of baculovirus vectors to BacMam vectors involves replacement of the viral specific expression cassette with a mammalian expression cassette or the addition of a mammalian expression cassette. Viruses are produced using standard methods in a few weeks. Mammalian cells transduced with the BacMam viruses have been routinely used as substitutes for stable cell lines.

Affiliation(s): (2) Department of Gene Expression and Protein Biochemistry, GlaxoSmithKline, King of Prussia, PA
(3) Department of Biological Reagents and Assay Development, GlaxoSmithKline, King of Prussia, PA

Book Title: Baculovirus and Insect Cell Expression Protocols

Series: Methods in Molecular Biology | Volume: 388 | Pub. Date: Jul-06-2007 | Page Range: 95-114 | DOI: 10.1007/978-1-59745-457-5_5

Subject: Microbiology

Key Words: BacMam - modified baculovirus - transient expression - viral transduction - gene over-expression - coexpression - GPCR - FACS

References

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