Methods for Gene Silencing With RNAi
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This chapter describes a technique for synthesizing and transfecting double stranded RNA (dsRNA) for RNA interference in Sf-21
cell culture. Transfection with dsRNA only requires 1 h and the cells are usually recovered within 12 h. Suggestions for designing
dsRNA are included in the methods. Furthermore, websites are provided for rapid and effective dsRNA design. Three kits are
essential for using the described methods: RNAqueous®-4PCR and MEGAscript™ T7 kit from Ambion and the Superscript? III kit
from Invitrogen.
Affiliation(s): (2) Department of Biological and Environmental Engineering, Cornell University, Ithaca, NY
(3) University of Maryland Biotechnology Institute and Department of Chemical Engineering, University of Maryland, College Park, MD
(3) University of Maryland Biotechnology Institute and Department of Chemical Engineering, University of Maryland, College Park, MD
Book Title: Baculovirus and Insect Cell Expression Protocols
Series: Methods in Molecular Biology | Volume: 388 | Pub. Date: Jul-06-2007 | Page Range: 427-433 | DOI: 10.1007/978-1-59745-457-5_23
Subject: Microbiology
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