Springer Protocols: Abstract: Alternative Bioreactor Strategy for Probing Infection and Production

Alternative Bioreactor Strategy for Probing Infection and Production

By: Yu-Chen Hu², William E. Bentley³

Abstract

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Probing the baculovirus infection process is essential in optimizing the recombinant protein production. Typically, researchers monitor the infection process in the stirred tank reactor, which, however, contains a population of cells infected at different times after virus inoculation. This chapter describes a two-stage reactor system consisting of an upstream continuous stirred tank reactor and a downstream tubular reactor with segmented plug flow for probing baculovirus infection and production.

Affiliation(s): (2) Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan
(3) University of Maryland Biotechnology Institute and Department of Chemical Engineering, University of Maryland, College Park, MD

Book Title: Baculovirus and Insect Cell Expression Protocols

Series: Methods in Molecular Biology | Volume: 388 | Pub. Date: Jul-06-2007 | Page Range: 419-425 | DOI: 10.1007/978-1-59745-457-5_22

Subject: Microbiology

Key Words: Tubular reactor - baculovirus infection - insect cell culture - monitoring

References

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6.	Hu, Y.-C., Wang, M.-Y., and Bentley, W. E. (1997) A tubular segmented-flow bioreactor for the infection of insect cells with recombinant baculovirus. Cytotechnology 24, 143–152.

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