Springer Protocols: Abstract: Monitoring and Visualization of Baculovirus Infection Using Green Fluorescent Protein Strategy

Monitoring and Visualization of Baculovirus Infection Using Green Fluorescent Protein Strategy

By: Hyung Joon Cha², William E. Bentley³

Abstract

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Through green fluorescent protein (GFP) strategy, facile and fast monitoring and visualization of baculovirus infection in insect cells is possible in vivo. This chapter describes two novel techniques for simple determination of virus titer in the baculovirus expression system using GFP coexpression and rapid monitoring of infection of Sf-9 insect cells using combination of GFP and early-to-late (ETL) promoter. It is anticipated that the use of GFP under the control of ETL promoter will facilitate vector construction, virus isolation, and titer determination.

Affiliation(s): (2) Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, Korea
(3) University of Maryland Biotechnology Institute and Department of Chemical Engineering, University of Maryland, College Park, MD

Book Title: Baculovirus and Insect Cell Expression Protocols

Series: Methods in Molecular Biology | Volume: 388 | Pub. Date: Jul-06-2007 | Page Range: 407-417 | DOI: 10.1007/978-1-59745-457-5_21

Subject: Microbiology

Key Words: Baculovirus infection - monitoring - green fluorescent protein - insect cell culture

References

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