Springer Protocols: Abstract: Transforming Lepidopteran Insect Cells for Improved Protein Processing

Transforming Lepidopteran Insect Cells for Improved Protein Processing

By: Robert L. Harrison², Donald L. Jarvis³

Abstract

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The lepidopteran insect cells used with the baculovirus expression vector system (BEVS) are capable of synthesizing and accurately processing foreign proteins. However, proteins expressed in baculovirus-infected cells often fail to be completely processed, or are not processed in a manner that meets a researcher’s needs. This chapter discusses a metabolic engineering approach that addresses this problem. Basically, this approach involves the addition of new or enhancement of existing protein processing functions in established lepidopteran insect cell lines. Methods for engineering these cell lines and assessing their properties as improved hosts for the BEVS are detailed. Examples of lepidopteran insect cell lines engineered for improved protein N-glycosylation and trafficking are described.

Affiliation(s): (2) USDA/ARS Insect Biocontrol Laboratory, Beltsville, MD
(3) Department of Molecular Biology, University of Wyoming, Laramie, WY

Book Title: Baculovirus and Insect Cell Expression Protocols

Series: Methods in Molecular Biology | Volume: 388 | Pub. Date: Jul-06-2007 | Page Range: 341-356 | DOI: 10.1007/978-1-59745-457-5_17

Subject: Microbiology

Key Words: Insect cells - baculovirus - baculovirus expression vector system - BEVS - cell transformation - genetic engineering - metabolic engineering - protein N-glycosylation - glycosyltransferase - protein folding - protein trafficking

References

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