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Useful Tools
Small-Scale Protein Production With the Baculovirus Expression Vector System
Abstract
Improved methods of baculovirus cloning and insect cell culture and their commercialization have made the use of the baculovirus expression vector system (BEVS) a routine tool for the production of preparative quantities of recombinant protein. This chapter outlines basic techniques for small-scale protein production using the BEVS, including protocols for expression from adherent and suspension insect cell cultures, titer estimation, and expression optimization.
Affiliation(s): (2) Molecular Probes Invitrogen Detection Technologies, Eugene, OR
Series: Methods in Molecular Biology  |  Volume: 388  |  Pub. Date: Jul-06-2007  |  Page Range: 211-223  |  DOI: 10.1007/978-1-59745-457-5_10
Subject:  Microbiology
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