SpringerProtocols: Abstract: Gastric Carcinogenesis and Helicobacter pylori Infection

10. Gastric Carcinogenesis and Helicobacter pylori Infection

By: Christine Varon³, Jean-François Mosnier⁴, Philippe Lehours⁵, Tamara Matysiak-Budnik⁶, Francis Mégraud⁵

Abstract

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Helicobacter pylori is the most frequent cause of infection-induced cancer worldwide. Gastric carcino-genesis is the consequence of the important and life-long inflammation induced by H. pylori in the stomach. Gastric carcinogenesis, can be studied in many ways. In this chapter, we focus on some aspects concerning the bacteria, and others concerning the host. On the bacterial side, the methods exploring the presence of the cag pathogenicity island including cagA and the consequences on epithelial cells are presented. On the host side, tissue microarray, immunohistochemistry and chromogenic in situ hybridization (CISH) are described.

Affiliation(s): (3) INSERM U853, Université Victor Segalen, Bordeaux, France
(4) PU-PH Pathology, INSERM U539, CHU de Nantes, Nantes, France
(5) MCU-PH Bacteriology, INSERM U853, Université Victor Segalen, Bordeaux, France
(6) MCU-PH, Institut des Maladies de l'Appareil Digestif, Hépato-Gastroentérologie, CHU de Nantes, Nantes, France

Book Title: Inflammation and Cancer: Methods and Protocols: Volume 1: Experimental Models and Practical Approaches

Series: Methods in Molecular Biology | Volume: 511 | Pub. Date: Sep-01-2008 | Page Range: 237-265 | DOI: 10.1007/978-1-59745-447-6_10

Subject: Cancer Research

Key Words: cagPAI - cagA - EPIYA - vacA - IL-8 - Intestinal metaplasia - Tissue microarray

References

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1.	Parkin, D.M. (2006) The global health burden of infection-associated cancers in the year 2002.International Journal of Cancer 118, 3030–3044

2.	Warren, J.R., and Marshall, B. (1983) Unidentified curved bacilli on gastric epithelium in active chronic gastritis.The Lancet 1, 1273– 1275

3.	IARC. (1994)In “Monographs on the evaluation of carcinogenic risks to humans” 61, 177–220

4.	Hill, A.B. (1965) The environment and disease: association or causation?Proceedings of the Royal Society of Medicine 58, 295–300

5.	Ekstrom, A.M., Held, M., Hansson, L., Engstrand, L., and Nyren, O. (2001)Helicobacter pylori in gastric cancer established by CagA immunoblot as a marker of past infection.Gas-troenterology 121, 784–791

6.	Wong, B.C.Y., Lam, S.K., Wong, W.M., et al. (2004)Helicobacter pylori eradication to prevent gastric cancer in a high-risk region of China – A randomized controlled trial.JAMA: Journal of the American Medical Association 291, 187–194

7.	Moss, S.F., Calam, J., Agarwal, B., Wang, S., and Holt, P.R. (1996) Induction of gastric epithelial apoptosis byHelicobacter pylori. Gut 38, 498–501

8.	Cahill, R.J., Sant, S., Beattie, S., Hamilton, H., and O'Morain, C. (1994)Helicobacter pylori and increased epithelial cell proliferation: a risk factor for cancer.European Journal of Gastro-enterology and Hepatology 6, 1123–1128

9.	Touati, E., Michel, V., Thiberge, J.M., Wuscher, N., Huerre, M., and Labigne, A. (2003) ChronicHelicobacter pylori infections induce gastric mutations in mice.Gastroenter-ology 124, 1408–1419

10.	Kim, J.J., Tao, H., Carloni, E., Leung, W.K., Graham, D.Y., and Sepulveda, A.R. (2002)Helicobacter pylori impairs DNA mismatch repair in gastric epithelial cells.Gastroenterol-ogy 123, 542–553

11.	Censini, S., Lange, C., Xiang, Z., et al. (1996)cag, a pathogenicity island ofHelicobacter pylori, encodes type I-specific and disease-associated virulence factors.Proceedings of the National Academy of Sciences of the United States of America 93, 14648–14653

12.	Viala, J., Chaput, C., Boneca, I.G., et al. (2004) Nod1 responds to peptidoglycan delivered by theHelicobacter pylori cag pathogenic-ity island.Nature Immunology 5, 1166–1174

13.	Higashi, H., Tsutsumi, R., Fujita, A., et al. (2002) Biological activity of theHelicobacter pylori virulence factor CagA is determined by variation in the tyrosine phosphorylation sites.Proceedings of the National Academy of Sciences of the United States of America 99, 14428– 14433

14.	Higashi, H., Tsutsumi, R., Muto, S., et al. (2002) SHP-2 tyrosine phosphatase as an intracellular target ofHelicobacter pylori CagA protein.Science 295, 683–686

15.	Backert, S., Moese, S., Selbach, M., Brinkmann, V., and Meyer, T.F. (2001) Phospho-rylation of tyrosine 972 of theHelicobacter pylori CagA protein is essential for induction of a scattering phenotype in gastric epithelial cells.Molecular Microbiology 42, 631–644

16.	Argent, R.H., Kidd, M., Owen, R.J., Thomas, R.J., Limb, M.C., and Atherton, J.C. (2004) Determinants and consequences of different levels of CagA phosphorylation for clinical isolates ofHelicobacter pylori.Gastroenterology 127, 514–523

17.	Cover, T.L, and Blaser, M.J. (1992) Purification and characterization of the vacuolating toxin fromHelicobacter pylori.Journal of Biological Chemistry 267, 10570–10575

18.	Atherton, J.C., Cao, P., Peek, R.M., Tum-muru, M.K.R., Blaser, M.J., and Cover, T.L. (1995) Mosaicism in vacuolating cytotoxin alleles ofHelicobacter pylori – Association of specific vacA types with cytotoxin production and peptic ulceration.Journal of Biological Chemistry 270, 17771–17777

19.	Rhead, J.L., Letley, D.P., Mohammadi, M., et al. (2007) A newHelicobacter pylori vacuolat-ing cytotoxin determinant, the intermediate region, is associated with gastric cancer.Gas-troenterology 133, 926–936

20.	Papini, E., Zoratti, M., and Cover, T.L. (2001) In search of theHelicobacter pylori VacA mechanism of action.Toxicon 39, 1757–1767

21.	Boquet, P., Ricci, V., Galmiche, A., and Gauthier, N.C. (2003) Gastric cell apoptosis andH. pylori: has the main function of VacA finally been identified?Trends in Microbiology 11, 410–413

22.	Fujikawa, A., Shirasaka, D., Yamamoto, S., et al. (2003) Mice deficient in protein tyrosine phosphatase receptor type Z are resistant to gastric ulcer induction by VacA ofHelicobacter pylori.Nature Genetics 33, 375–381

23.	Gerhard, M., Lehn, N., Neumayer, N., et al. (1999) Clinical relevance of theHelicobacter pylori gene for blood-group antigen-binding adhesin.Proceedings of the National Academy of Sciences of the United States of America 96, 12778–12783

24.	Correa, P., Haenszel, W., Cuello, C., et al. (1990) Gastric precancerous process in a high risk population: cohort follow-up.Cancer Research 50, 4737–4740

25.	Matsukura, N., Suzuki, K., Kawachi, T., et al. (1980) Distribution of marker enzymes and mucin in intestinal metaplasia in human stomach and relation to complete and incomplete types of intestinal metaplasia to minute gastric carcinomas.Journal of National Cancer Institute 65, 231–240

26.	Halliwell, B. (2007) Oxidative stress and cancer: have we moved forward?The Biochemical Journal 401, 1–11

27.	Kang, G.H., Lee, H.J., Hwang, K.S., Lee, S., Kim, J.H., and Kim, J.S. (2003) Aberrant CpG island hypermethylation of chronic gastritis, in relation to aging, gender, intestinal metaplasia, and chronic inflammation.American Journal of Pathology 163, 1551–1556

28.	Kountouras, J., Zavos, C., and Chatzopoulos, D. (2005) New concepts of molecular biology on gastric carcinogenesis.Hepato Gastroenter-ology 52, 1305–1312

29.	Zavros, Y., Eaton, K.A., Kang, W., et al. (2005) Chronic gastritis in the hypochlorhydric gas-trin-deficient mouse progresses to adenocarci-noma.Oncogene 24, 2354–2366

30.	Occhialini, A., Marais, A., Urdaci, M., et al. (2001) Composition and gene expression of thecag pathogenicity island inHelicobacter pylori strains isolated from gastric carcinoma and gastritis patients in Costa Rica.Infection and Immunity 69, 1902–1908

31.	Santos A, Queiroz DM, Ménard A, et al. (2003) New pathogenicity marker found in the plasticity region of theHelicobacter pylori genome.Journal of Clinical Microbiology 41, 1651–1655

32.	Rudi, J., Kolb, C., Maiwald, M., et al. (1998) Diversity ofHelicobacter pylori vacA andcagA genes and relationship to VacA and CagA protein expression, cytotoxin production, and associated diseases.Journal of Clinical Microbiology 36, 944–948

33.	Argent, R.H., Zhang, Y.L., and Atherton, J.C. (2005) Simple method for determination of the number ofHelicobacter pylori CagA variable-region EPIYA tyrosine phosphorylation motifs by PCR.Journal of Clinical Microbiology 43, 791–795

34.	Rice, P., Longden, I., and Bleasby, A. (2000) EMBOSS: the European Molecular Biology Open Software Suite.Trends in Genetics 16, 276–277

35.	Corpet, F. (1988) Multiple sequence alignment with hierarchical clustering.Nucleic Acids Research 16, 10881–10890

36.	Huang, X., and Miller, M. (1991) A time-efficient, linear-space local similarity algorithm.Advances in Applied Mathematics 12, 337–357

37.	Cover TL, Blanke SR, Hehcobaoter VacA, a paradigm per toxin multifunctionality Nat. Rev. microbial 2005; 3:320–32

38.	Atherton, J.C., Cover, T.L., Twells, R.J., Morales, M.R., Hawkey, C.J., and Blaser, M.J. (1999) Simple and accurate PCR-based system for typing vacuolating cytotoxin alleles ofHeli-cobacter pylori.Journal of Clinical Microbiology 37, 2979–2982

39.	Oosterhuis, J.W., Coebergh, J.W., and van Veen, E.B. (2003) Tumour banks: well-guarded treasures in the interest of patients.Nature Reviews Cancer 3, 73–77

40.	Kononen, J., Bubendorf, L., Kallioniemi, A., et al. (1998) Tissue microarrays for high-throughput molecular profiling of tumor specimens.Nature Medicine 4, 844–847

41.	Bezieau, S., Devilder, M.C., Avet-Loiseau, H., et al. (2001) High incidence of N and K-Ras activating mutations in multiple myeloma and primary plasma cell leukemia at diagnosis.Human Mutation 18, 212–224

42.	Hasegawa, Y., Takeda, S., Ichii, S., et al. (1995) Detection of K-ras mutations in DNAs isolated from feces of patients with colorectal tumors by mutant-allele-specific amplification (MASA).Oncogene 10, 1441–1445

43.	Jarry, A., Masson, D., Cassagnau, E., Parois, S., Laboisse, C., and Denis, M.G. (2004) Real-time allele-specific amplification for sensitive detection of the BRAF mutation V600E.Molecular and Cellular Probes 18, 349–352

44.	Laboisse, C.L., Augeron, C., Couturier-Turpin, M.H., Gespach, C., Cheret, A.M., and Potet, F. (1982) Characterization of a newly established human gastric cancer cell line HGT-1 bearing histamine H2-receptors.Cancer Research 42, 1541–1548

45.	Ferreira-Chagas, B., Lasne, G., Dupouy, S., et al. (2007) In vitro proinflammatory properties ofHelicobacter pylori strains causing low-grade gastric MALT Lymphoma.Helicobacter 12, 616–617

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