SpringerProtocols: Abstract: Skeletal (“Mesenchymal”) Stem Cells for Tissue Engineering

Skeletal (“Mesenchymal”) Stem Cells for Tissue Engineering

By: Pamela Gehron Robey³, Sergei A. Kuznetsov³, Mara Riminucci⁴, Paolo Bianco⁵

Abstract

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Skeletal stem cells (SSCs, commonly referred to as “mesenchymal” stem cells) are found in the bone marrow stromal cell (BMSC) fraction of post-natal bone marrow. They can be isolated in culture as adherent, clonogenic cells endowed with the ability to grow and differentiate into multiple lineages, all of which correspond to tissues that are integral parts of the skeleton. The multipotency of SSCs is probed by in vivo transplantation assays. The ability of SSCs to generate a cell strain competent to form significant amounts of bone in vivo has led to the formulation of preclinical models of bone repair.

Affiliation(s): (3) Department of Health and Human Services, Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD
(4) Department of Experimental Medicine, University of L’Aquila, L’Aquila, Italy, and San Raffaele Biomedical Science Park, Rome, Italy
(5) Department of Experimental Medicine and Pathology, Department of Health and Human Services, Craniofacial and Skeletal Diseases Branch, La Sapienza University, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, L’Aquila, Italy, and San Raffaele Biomedical Science Park, Rome, Italy

Book Title: Tissue Engineering

Series: Methods in Molecular Medicine | Volume: 140 | Pub. Date: Jul-27-2007 | Page Range: 83-99 | DOI: 10.1007/978-1-59745-443-8_5

Subject: Biochemistry

Key Words: Skeletal stem cells - Mesenchymal stem cells - Osteogenesis - Bone repair - Tissue engineering - Bone - Cartilage

References

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