SpringerProtocols: Abstract: Human Articular Chondrocytes Culture

Human Articular Chondrocytes Culture

By: Andrea Barbero³, Ivan Martin

Abstract

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In this chapter, we describe a strategy to expand adult human articular chondrocytes in monolayer while maintaining high post-expansion chondrogenic capacity. This method relies on the use of a specific growth factor cocktail during the two-dimensional (2D) propagation of chondrocytes. In addition, a protocol to isolate and grow single-colony-derived strains of chondrocytes is presented as a model to study the biology of different chondrocyte subpopulations.

Affiliation(s): (3) Institute for Surgical Research and Hospital Management, University Hospital Basel, Basel, Switzerland

Book Title: Tissue Engineering

Series: Methods in Molecular Medicine | Volume: 140 | Pub. Date: Jul-27-2007 | Page Range: 237-246 | DOI: 10.1007/978-1-59745-443-8_13

Subject: Biochemistry

Key Words: Chondrocyte - Cartilage - Growth factor - Cell expansion - Clone

References

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