SpringerProtocols: Abstract: Poly(?-amino esters): Procedures for Synthesis and Gene Delivery

4. Poly(β-amino esters): Procedures for Synthesis and Gene Delivery

By: Jordan J. Green¹, Gregory T. Zugates¹, Robert Langer¹, Daniel G. Anderson¹

Abstract

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Non-viral gene delivery systems are promising as they avoid many problems of viral gene therapy by having increased design flexibility, high safety, large DNA cargo capacity, and ease of manufacture. Here, we describe the use of polymeric vectors, in particular biodegradable poly(β-amino esters) (PBAEs), for non-viral gene delivery. These polymers are able to self-assemble with DNA and form positively charged gene delivery nanoparticles. Methods for synthesis of these polymers, particle self-assembly, and transfection using these particles are delineated. A standard protocol is presented as well as a high-throughput screening technique that can be used to more quickly optimize transfection parameters for efficient delivery.

Affiliation(s): (1) Department of Biological Engineering, Department of Chemical Engineering, and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA

Book Title: Macromolecular Drug Delivery: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 480 | Year: 2009 | Page Range: 1-11 | DOI: 10.1007/978-1-59745-429-2_4

Subject: Pharmacology/Toxicology

Key Words: Gene therapy - Drug delivery - Polymers - Transfection - High-throughput screening

References

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